SCIENTIFIC-TECHNICAL PROGRAM

IRN BR24992903 «Practical implementation of modern molecular genetic, physiological, biochemical, biotechnological methods and digital phenotyping in breeding of economically important agricultural crops».

Задачи программы
Широкомасштабное фенотипирование биохимических, морфологических,
иммунологических и физиологических признаков, качественных показателей
сельскохозяйственных культур с использованием высокопроизводительных цифровых, хроматографических и инструментальных методов.
Идентификация QTL и генов-кандидатов экономически значимых
сельскохозяйственных культур, связанных с селекционно-ценными признаками на основе полногеномного анализа ассоциаций (GWAS). Разработка новых, информативных SNP- маркеров на основе GWAS.
Использование комплекса современных молекулярных маркеров на всех этапах практической селекции пшеницы, ячменя, кукурузы, сои, гороха и выделение перспективных линий с ценными генами селекционных признаков.
Отбор высокопродуктивных ценных форм пшеницы, ячменя, кукурузы, сои,
гороха на всех этапах селекционного процесса по признакам продуктивности, жирно-кислотному, аминокислотному составу, питательной ценности для конечного типа использования (продукты питания, корма, масложировая продукция и др.).
Ускоренная селекция зерновых культур, основанная на гомозиготизации ценного селекционного материала и получения дигаплоидных линий.
Решение узких мест селекции само- и перекрестноопыляющихся культур на
этапах гибридизации и контроля гетерозиготности гибридов F1.
Создание, комплексный анализ и передача на госсортоиспытание перспективных линий экономически значимых сельскохозяйственных культур.
Интеграция новых инструментов геномики, феномики, селекции и культуры
тканей в практические селекционные проекты.

Результаты за 2024 г.:


1.Сформированы коллекции трех зерновых культур (мягкая пшеница, ячмень, кукуруза) и двух зернобобовых культур (горох и соя).
2.Начато проведение широкомасштабного фенотипирования морфологических, иммунологических и физиологических признаков сельскохозяйственных культур с использованием высокопроизводительных цифровых методов.
3.Проведен отбор перспективных линий зерновых и зернобобовых культур на основе полевых опытов.
4.Выделена ДНК гибридных комбинаций мягкой пшеницы, ячменя, сои и гороха, начаты работы по генотипированию ДНК-маркерами.
5.Начаты работы по ускоренной селекции ячменя и пшеницы.

Results for 2025:


During the second year of implementing the Program’s objectives and activities, studies
were continued:

  • on large-scale phenotyping of barley, bread wheat, maize, soybean, and pea
collections;
  • genotyping of collections using chip-based next-generation sequencing
technologies (Illumina, Affymetrix) and KASP, Amplifluor, and ASQ DNA-
marking technologies;
  • identification of QTLs associated with valuable traits using GWAS methodology;
  • traditional methods of breeding, morphophysiology, biochemistry, and
immunology, as well as modern approaches of genomics, biotechnology, and
bioinformatics were applied at various stages of practical breeding.
  • Promising genotypes/lines with valuable genes and favorable alleles associated
with important breeding traits were identified and transferred for further
acceleration and improvement (doubled haploids, SSD, Speed breeding) of
breeding programs and seed production.
  • Promising lines of barley and wheat, identified as a result of the scientific
groundwork and the current Program, have undergone various stages of testing,
the best of which have been transferred to State Cultivar Trials - cultivars of
spring barley (3) and soft wheat (2), created as a result of ongoing comprehensive
research by the Program participants.
Both individual scientists and research teams from the co-executing organizations participated
in the implementation of the tasks, stages, and activities of the Program:
1. RSE on REM “Institute of Plant Biology and Biotechnology,” CS MESHE of the
Republic of Kazakhstan;
2. LLP “Karabalyk AES,” Kostanay region;
3. LLP “A.I. Barayev Research and Production Center of Grain Farming,” Akmola region;
4. LLP “North Kazakhstan AES,” North Kazakhstan region;
5. LLP “A.F. Khristenko Karaganda AES,” Karaganda region;
6. LLP “I. Zhakhaev Kazakh Research Institute of Rice Growing,” Kyzylorda region;
7. LLP “Krasnovodopad AES,” Turkistan region;
8. NAO “S. Seifullin Kazakh Agrotechnical Research University,” Astana;
9. NAO “Ilyas Zhansugurov Zhetysu University,” Zhetysu region, Taldykorgan;
10. NAO “al-Farabi Kazakh National University,” Almaty;
11. LLP “National Center for Biotechnology (NCB),” Astana;
12. RSE on REM “Research Institute for Biological Safety Problems” Farm “Sabyr”.
Candidate of Biological Sciences, Professor, Academician of the National Academy of
Sciences under the President of the Republic of Kazakhstan – 1; Doctor of Biological Sciences,
Professor – 2; Doctor of Agricultural Sciences, Professor – 1; Doctor of Geographical Sciences,
Professor – 1; Candidate of Agricultural Sciences, Associate Professor – 2; Candidates of

Agricultural Sciences – 5; PhD, Associate Professor – 1; PhD – 7; Higher Education – 20;
Masters – 6; Doctoral students – 4; Master’s students – 2; Bachelors – 3.
As part of the implementation of the Program, Coordination meetings were held both
online and offline throughout the year, as well as Field Days and experimental trials evaluation
at the A.I. Barayev Research and Production Center of Grain Farming and Karabalyk AES, and
a workshop at I. Zhansugurov Zhetysu University and School for Young Plant Breeders. All
reports from the co-executing organizations were presented at the Scientific Councils of the
respective institutions and during the online Coordination meeting held on October 3, 2025 and
offline December 8, 2025. Master’s and doctoral students from al-Farabi Kazakh National
University, S. Seifullin Kazakh Agrotechnical Research University, L.N. Gumilyov Eurasian
National University, and Zhetysu University named after I. Zhansugurov participated in the
work as members of the research teams of these co-executing organizations. International
scientists participated in the Program on a voluntary basis, including Professor Simon Griffiths
(John Innes Centre, United Kingdom).

Information for potential users

As part of this program, KASP marker sets were developed for genotyping cereals and

legume crops associated with traits of adaptability, yield, disease resistance, and grain quality.

Specialized marker sets for the main crops were developed for each study region. Based on the

results obtained during the Program, we offer genotyping services for cereals and legume crops

to breeding centers, research organizations, and agricultural specialists. The use of the

developed KASP markers allows for the rapid identification of the most promising lines and

accelerates the breeding process.

Publications for 2024
Publications for 2025
© Institute of Plant Biology and Biotechnology
+7 (727) 394-75-62 ipbbkz@gmail.com